HYBRID EVENT: You can participate in person at Rome, Italy or Virtually from your home or work.

3rd Edition of Euro-Global Conference on Biotechnology and Bioengineering

June 14-15 | Hybrid Event

June 14-15, 2023 | Rome, Italy
ECBB 2022

Christoph Koppl

Christoph Koppl, Speaker at Biotechnology Conferences 2022
Austrian Centre of Industrial Biotechnology GmbH, Austria
Title: Combinatorial fusion tag yields powerful platform process for the production of pharmaceutically relevant proteins

Abstract:

Expression of complex recombinant proteins in E. coli is a major challenge in industrial biotechnology as the protein of interest is often produced with very low titres or misfolded as inclusion body. One possibility to circumvent these problems is the use of fusion partners. Unfortunately, fusion tags can vary greatly in their effectiveness, which is often dependent on the protein of interest. We found that a solubility tag originating from the gene 10 of the T7 bacteriophage can greatly increase recombinant protein titres of multiple relevant biopharmaceutical proteins. Due to its small size of only 22 amino acids, this fusion tag keeps the exerted additional stress on the cellular transcription machinery on a minimal level. The effectiveness of the fusion tag has been evaluated in carbon limited laboratory scale fed-batch fermentations. There, specific recombinant protein titres could be increased by a factor of greater than two compared to expression of the native protein. Since the fermentations were performed under identical conditions, this effect can be attributed solely to the fusion tag which was fused to the N-terminus of the proteins of interest. Furthermore, this fusion tag can be coupled with a pentapeptide cleavage site, resulting in highly efficient and specific cleavage by a human caspase-2 variant. The cleavage is largely independent of the N-terminal amino acid of the protein of interest, which makes this system universally applicable. The combination of the expression enhancing fusion tag with the caspase-2 protease system for efficient tag removal yields a highly capable system to produce challenging recombinant proteins in E. coli, offering an attractive production platform for biopharmaceutical industry.

Biography:

Dipl.-Ing. Christoph Köppl graduated from the master’s program Biotechnology at the University of Natural Resources and Life Sciences, Vienna as Dipl.-Ing. in 2020. Shortly after, he joined the research group of Univ. Prof. Dr. Gerald Striedner at the Department of Biotechnology (University of Natural Resources and Life Sciences, Vienna). He is currently enrolled in the doctoral study program Bioprocess Engineering (University of Natural Resources and Life Sciences, Vienna) and started his doctoral thesis at the Austrian Centre of Industrial Biotechnology, Vienna in October 2021. So far, he has contributed to three research articles published in SCI(E) journals as co-author.
 

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