Title: Time to change to time: Single amino acid resolution anti-tumor platform Tag-Tack is standby
Abstract:
In order to shorten drug’s evolution time than personal cancer’s evolution time, a Tag-Tack platform was designed and constructed to make introcytoplastic missense proteins to be druggable in 6 months.
We screened 8 human ScFVs for 6 introcytoplastic missense proteins?TP53 p.R248Q, KRAS p.G13D, BRAF p.V600E, IDH1 p.R132C, PIK3CA p.E545K, EML4-ALK v3a fusion?, and cloned them into Tag-tack platform to be Tag-Tacker. 6 cell-lines harboring the former 6 introcytoplastic missense proteins were target killed by those 8 Tag-Tackers, while no tagging protein cell-lines were alive. Based on those in vtro cell line’s results, an out of treatment patient having BRAF p.K601E mutation was successfully treatmented by lesion injection of BRAF p.K601E Tagtacker’s Compassionate using.
Tag-tack, a single amino acid resolution anti-tumor platform Which can make any introcytoplastic missense proteins to be druggable in 6 months is standby!
