Euro-Global Conference on Biotechnology and Bioengineering

September 06-08, 2021 | Online Event

September 06-08, 2021 | Online Event
ECBB 2021

Olajumoke Oyebode

Speaker at Biotechnology and Bioengineering 2021 - Olajumoke Oyebode
University of Johannesburg, South Africa
Title : Influence of photobiomodulation at 830 nm on proliferation and differentiation in diabetic wounded human skin fibroblast cells

Abstract:

This study investigated the effect of photobiomodulation (PBM) using near infra-red light (830 nm) on cell proliferation and differentiation in two human fibroblast cell models namely, normal wounded (NW) and diabetic wounded (DW). The cells were irradiated at a wavelength of 830 nm with a fluence of 5 J/cm2 which resulted in an irradiation time of 430 s. Control cells were not irradiated (0 J/cm2). Thereafter, cell was incubated for 24 and 48 h and cellular viability was assessed by the trypan blue exclusion test and Apotox-glo triplex assay. Cell proliferation was investigated using bromodeoxyuridine (BrdU) proliferation assay. The release of transforming growth factor beta-1 (TGF-β1; TGF-β1 is a growth factor released during the process of wound repair and it is involved in the activation of fibroblasts in the process of trans-differentiation, a precursor to differentiation while α-SMA is also involved in the differentiation process) and p-Smad2/3 was ascertained using ELISA, while immunofluorescence was also used to observe the presence of the myofibroblast marker alpha smooth muscle actin (α-SMA). In comparison with the control groups, PBM significantly increased cell viability and proliferation. Although there were no significant changes in p-Smad2/3 over time, DW cells showed a moderate increase in TGF-β1. As incubation time increased, there was an increase in fluorescence of α-SMA in DW cells. This study shows that NW and DW cells responded positively to PBM at 830 nm, and PBM produced a stimulatory effect on cell viability, proliferation and differentiation to initiate wound healing in DW cells in vitro.

Biography:

Dr. Olajumoke Oyebode attended the University of Ibadan, Nigeria where she graduated with an MSc degree in Biochemistry. She then proceeded to the University of KwaZulu-Natal, South Africa where she graduated in 2019 with a PhD degree in Biochemistry under the supervision of Prof. MS Islam. In 2020, she joined the research group of Prof. Abrahamse at the Laser Research Centre of the University of Johannesburg, South Africa as a postdoctoral fellow under the supervision of Prof. Houreld. She has published extensively in internationally accredited high impact factor journals.

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