HYBRID EVENT: You can participate in person at Rome, Italy or Virtually from your home or work.

3rd Edition of Euro-Global Conference on Biotechnology and Bioengineering

June 14-15 | Hybrid Event

June 14-15, 2023 | Rome, Italy
ECBB 2021

Marzena Mazurek

Marzena Mazurek, Speaker at Biotechnology Conference
University of Rzeszow, Poland
Title: Monitoring of clonal fidelity of highbush blueberry cultures propagated by axillary and adventitious shoots


Plant tissue culture technology is being widely used for large scale highbush blueberry plant multiplication. The main aim of micropropagation is the clonal production of 'true-to-type' plants. However, in vitro conditions induce somaclonal variations which can affect the undesired morphological and genetic changes of regenerated plants. Somaclonal variation, either genetic and epigenetic or combination of both, is influenced by the genotype, explant type, culture medium or age of the donor plants. The occurrence of callus and adventitious shoots during the in vitro culture of blueberries is mainly considered as a source of somaclonal variation. In blueberry in vitro cultures, adventitious shoots occur spontaneously and are common. To monitor clonal fidelity the differences between the in vitro cultures of the highbush blueberry ‘Brigitta blue’ plants derived from axillary (Ax) and adventitious shoots (Ad) using as an explant, were investigated. The two types of culture: the axillary shoots derived cultures (Ax-TC) and the adventitious shoots derived cultures of highbush blueberries plants (Ax-TC) were analysed during 3 subsequent passage. The analyses were carried out in the determination of morphological and molecular differences between Ax-TC and Ax-TC cultures. On the morphological level, the frequency and the length of the axillary shoots and adventitious ones were determined. Molecular studies were based on the methylation-sensitive amplification polymorphism (MSAP) technique. We indicated significant differences in studied traits like the ratio of Ax shoots in culture and the number of cultures that developed exclusively Ax shoots. Additionally, short adventitious shoots (< 1,5 cm in length) in Ad-TC cultures occurred more frequently in comparison to Ax-TC. What is more, different DNA methylation status among studied plants was revealed.


Marzena Mazurek studied Biotechnology at the University of Silesia in Katowice (Poland) and graduated as MS in 2008. Then She has started work at the University of Rzeszów in the assistant position. She is concluding a Ph.D. thesis entitled “Somaclonal changes of highbush blueberries plants propagated traditionally and in vitro method. She has published 2 research articles in SCI(E) journals and took part in two conferences.