Euro-Global Conference on Biotechnology and Bioengineering

September 06-08, 2021 | Online Event

September 06-08, 2021 | Online Event
ECBB 2021

Poonam Kumari

Speaker at Biotechnology and Bioengineering 2021 - Poonam Kumari
National Institute of Pharmaceutical Education & Research (NIPER), India
Title : Sortase A mediated peptide recruitment as a potential anti-virulence treatment for Staphylococcus aureus infection

Abstract:

Sortase A is a transpeptidase enzyme that is majorly present onto the cell wall of Gram-positive bacteria. It recognized and displays proteins on the cell wall via a conserved motif LPXTG at C- terminal. These proteins plays a major role in the virulence of the bacteria. Cell surface proteins not only helps bacteria to enter the host cells but also helps the bacteria to evade the immune systems using various mechanisms. In this study we have used a foreign peptide having LPETG motif at the C-terminal to redecorate the cell wall of the bacteria. Here, we used the approach to study the bacterial response after the recruitment of Foreign peptide onto the fibronectin binding, complement system activations and the pathogenesis of the bacteria. Here, we have observed a significant decrease in the binding of the Staphylococcus aureus to fibronectin by which bacteria enter in the host cells. As well as we have also observed a increase in the phagocytosis of the bacteria after the peptide treatment in-vitro. The present work provides a novel method to combat the Gram-positive infection by attenuating its virulence.

Biography:

Miss Poonam Kumari completed here Master in technology and currently pursuing her PhD from NIPER Guwahati, India. Her Ph.D. work is on “Sortase based biomolecular engineering”. During the last one year, she was able to develop a method for the generation of novel protein coding gene libraries, for which an Indian Patent application has been filed. She has screened the surface of S. aureus for evolving novel peptides that bind its surface with high affinity. Apart from her expertise in molecular biology tools set, she also has learned solid phase peptide synthesis, which is an asset for a Biology student. She has synthesized her peptides and analysed them for antibacterial activity. Her work has generated enough results, on which we are about to file a patent and communicate a manuscript from her last one-year work. She has published one research paper also from her thesis work and few are in communication.

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