Title: The potential of laccase produced from fungi for biotransformation of polycyclic aromatic hydrocarbons
Abstract:
A total of 804 fungi isolate were subjected to the screening of high laccase activity by four different indicators test, Poly R-478, RBBR, guaiacol, and syringaldazine. The results presented the diversity of 6 order, belonging to 17 families, 25 genera and 29 species, and most of the isolated strain was reacted with laccase chemical indicators and categorized as saprophytic, parasitic and ectomycorrhizal. Three fungi (Coriolopsis caperata, Fomes fomentarius, Pluteus chysophaeus) that isolated from Niah National Park and Lambir Hill National Park were selected for further experiment due to their high positive reaction on all tested indicators. The maximum level on the laccase production during fungi exponential growth was impacted by the addition of carbon source. Diphenic acid, 1-hydroxy-2napthoic acid, catechol, and salicylic acid, anthraquinone, benzoic acid, and phthalic acid were detected as metabolites of phenanthrene, anthracene, and pyrene.
