HYBRID EVENT: You can participate in person at London, UK or Virtually from your home or work.

5th Edition of Euro-Global Conference on Biotechnology and Bioengineering

September 18-20 | Hybrid Event

September 18-20, 2025 | London, UK
ECBB 2025

Screening extracts of fungal endophytes isolated from Allophylus abyssinicus (Hochst. Radlk.) for control of bean anthracnose

Hellen Gathiru Kariuki, Speaker at Biotechnology Conference
CIFOR-ICRAF, Kenya
Title: Screening extracts of fungal endophytes isolated from Allophylus abyssinicus (Hochst. Radlk.) for control of bean anthracnose

Abstract:

Common bean is consumed in large quantities globally but it is highly susceptible to Colletotrichum lindemuthianum, leading to 90 to 100% yield losses. This study investigated the bioactivity of secondary metabolites from Allophylus abyssinicus fungal endophytes against C. lindemuthianum, causing bean anthracnose. A total of 37 fungal endophytes were isolated from the leaves, bark and roots of A. abyssinicus and 20% identified by ITS-rDNA sequence analysis as Aspergillus hancockii, Penicillium christenseniae, Penicillium atrosanguineum and Penicillium manginii. Thirty-three of the endophytes were active against C. lindemuthianum in the dual culture assay with the highest inhibition being 82.6% (A. hancockii). Two of the most active endophytes (A. hancockii and P. christenseniae) were fermented on rice media and their methanol extracts partitioned between ethyl acetate and hexane. Aspergillus hancockii crude hexane extract had the highest inhibition (19.0±1.7mm at 100 mg/ml) against the pathogen under the bioassay screening and 6.3±3.8 mm at 6.25% against C. lindemuthianum under the minimum inhibitory concentration (0.625 mg/ml) screening. Chemical screening of the extracts revealed presence of alkaloids in A. hancockii and P. christenseniae; sterols, trite lindemuthianum rpenes and coumarins in A. hancockii. These results indicated that fungal endophytes from A. abyssinicus are a source of active compounds that can be used to control C. affecting common bean.

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