Title: Green synthesis and treatment of silver nanoparticles from Leishmania major in Iraq
Abstract:
Nanoparticles (NPs) play an important role in the diagnosis and treatment of diseases in consequence of their larger surface areas in comparison to the bulk material. Among the variety of nanomaterials, metal nanoparticles (MNPs) present unique physical, chemical and biological properties. The present study was evaluated the anti-Leishmania effect of silver nanoparticles on Leishmania major based on investigation of their action on various cellular parameters of the parasite.
Silver nanoparticles were synthesized using tannic acid (TA) as both reducing and stabilizer and monitored by UV–vis absorption spectroscopy and scanning electron microscopy (SEM) images that showed formation of spherical AgNPs ranging from 5 to 24 nm diameter.
TA coating resulted in a red-shift and broadening of bands. In vitro assays of Leishmania major promastigotes showed an increase of anti-leishmanial activity for AgNPs -TA. This screening can contribute to the development of new, green, and fast produced drugs aiming at leishmaniasis treatment. Moreover these biologically synthesized nanoparticles were found to be highly effective against different multi-drug resistant human pathogens.
Silver nanoparticles synthesized using tannic acid have potential application as an anti-leishmanial treatment. Furthermore, the use of natural compounds involves to the development of clean, nontoxic, biocompatible and environmentally benign methods to synthesize noble metal nanoparticles.
Conclusion: An eco-friendly route for silver NPs by TA was demonstrated for anti-leishmanial activity. A one-container synthesis based on chemical reduction was used to prepare silver NPs. TA gains an attention as it is a naturally derived polyphenolic compound and due to its unique biological properties. UV–vis absorption spectroscopy and scanning electron microscopy (SEM) images that showed formation of spherical AgNPs ranging from 5 to 24 nm diameter.
Samples collection → Culture →PCR →Synthesis of Ag-NPs → UV–vis spectroscopy and SEM
