HYBRID EVENT: You can participate in person at Rome, Italy or Virtually from your home or work.

3rd Edition of Euro-Global Conference on Biotechnology and Bioengineering

June 14-15 | Hybrid Event

June 14-15, 2023 | Rome, Italy
ECBB 2022

Lactococcus lactis, a promising cell factory to functionally express membrane proteins

Frelet Barrand Annie, Speaker at Bioengineering Conferences
Institute FEMTO, France
Title: Lactococcus lactis, a promising cell factory to functionally express membrane proteins


Membrane proteins (MPs) play key roles in most crucial cellular processes ranging from cell to cell communication to signaling processes. Despite recent improvements, the expression of functionally folded membrane proteins in sufficient amounts for their functional and structural characterization remains a challenge. Indeed, it is still difficult to predict whether a protein can be overproduced in a functional state in some expression system(s). Prokaryotic expression systems present several advantages over eukaryotic ones. Among them, Lactococcus lactis (L. lactis) has emerged in the last two decades as a good alternative expression system to E. coli. The purpose of this presentation is to describe L. lactis and its tightly inducible system, NICE, for the effective expression of membrane proteins from both prokaryotic and eukaryotic origins.


Dr. Annie FRELET-BARRAND studied biochemistry at the University of Franche-Comté (France) and was graduated as MS in 1998. In 2006, she received her PhD degree on MPs characterization at the Institute of Plant Biology, Zurich. During her postdoctoral fellowship (CEA Grenoble, France), she developed L. lactis system for functional characterization of MPs. In 2009, she became CNRS Researcher at CEA Saclay, studying MPs involved in liver detoxification. In 2015, she integrated the Institute FEMTO-ST and is now studying on biochemical and biophysical ways to study proteins and other biological elements. She published 19 research articles and 4 book chapters (h=14).