Title: Analysis of molecular features and therapeutic potential of l-asparaginase from a Marine bacterium
L-asparaginase is an antileukemic enzyme that is majorly utilized in the treatment of acute lymphoblastic leukaemia (ALL) and other blood cancers. A number of groups have made efforts to explore this vital agent. However, the use of L-asparaginase has been observed to have several side effects. Moreover, recurrent dosages of this form of the enzyme are required due to its shorter half-life in blood circulation, thus limiting its clinical efficacy and increasing treatment cost. Thus there are vigorous efforts undergoing to look for L-asparaginase with improved features. The work explores an extracellular glutaminase-free L-asparaginase-producing Bacillus australimaris NJB19 from marine resources. A number of parameters have been analyzed to find the optimal levels for achieving increased yields of enzyme production. Further, the biochemical analysis of the protein was conducted for finding out optimum parameters, Km and Vmax. The structural analysis denotes that the protein was type II Lasparaginase with no toxin domain. Furthermore, the cytotoxicity of the produced Lasparaginase was assessed using three solid tumor cell lines employing MTT and LDH assays. The cytotoxicity evaluation demonstrates the highest cytotoxic activity for MCF-7 and to MDAMB231 and DU-145 cells. Therefore, glutaminase-free L-asparaginase from marine B. australimaris NJB19 appears to be of potential value to be exploited commercially for ALL treatments.
Audience Take Away Notes :
- The presentation will cover the various aspects of L-asparaginase particularly how marine resources could be useful in looking for therapeutically significant enzymes particularly here L-asparaginase. The various biochemical and molecular approaches to analyze this enzyme will also be deliberated.
- The audience will learn the approaches to look for novel enzymes. They would also learn the distinct parameters and also the technical details that would let them understand the characteristic features of a novel enzyme. Learning and understanding these details would enable them to use it for their research and development work.
- Attending this presentation will help the audience in designing their approaches for an improved outcome of the research objectives that they are involved into.
- The outcomes of this presentation will help faculty and scientists to take up their research and teaching in an effective and meticulous manner