HYBRID EVENT: You can participate in person at Rome, Italy or Virtually from your home or work.

4th Edition of Euro-Global Conference on Biotechnology and Bioengineering

September 19-21 | Hybrid Event

September 19-21, 2024 | Rome, Italy
ECBB 2023

Gopal Prasad Agarwal

Gopal Prasad Agarwal, Speaker at Bioengineering Conference
Indian Institute of Technology Delhi, India
Title: Nanofiltration mediation for efficient production of second generation bioethanol by wild-type yeast from Lignocellulosic hydrolysate


Nanofiltration, a relatively new synthetic membrane-based separation process was first used to fractionate lignocellulosic hydrolysate. The fractionated streams of permeate and retentate were fermented separately to produce bioethanol in high yield. The choice of the right nanofiltration membrane was critical to achieving the optimal separation of glucose and xylose of the lignocellulosic hydrolysate. The molecular weight cut-off for the nanofiltration membrane was between 100 to 300 and the maximum transmembrane pressure used was 30 bar. The volumetric flux of these membranes was in the range of 60 to 100 LMH (liter m-2 h-1) for transmembrane pressure > 20 bar. These nanofiltration membranes were procured from various manufacturers like Novasep USA, Snyder USA and Permionics India. The separated streams thus obtained were completely fermented via wild-type yeasts namely Saccharomyces cerevisiae and Pichia stiptis into ethanol. The intervention of nanofiltration played an important role whereby the use of recombinant strain was avoided. Also, the complete utilization of carbohydrates of lignocellulosic hydrolysate was obtained. Nanofiltration not only separated the 5-carbon and 6-carbon carbohydrates but also separated toxins of the hydrolysate into the stream which was fermented by Saccharomyces cerevisiae. The productivity of ethanol was 2 to 3 times faster in the present process than the conventional method which were in use so far. In a nanofiltration-assisted process, high sugar concentrations as high as 100 g/l could be fermented to its completion


Dr. Gopal P. Agarwal was a Professor at Indian Institute of Technology Delhi, INDIA for over 3 decades. He was teaching a course on Applications of membranes in Bioprocessing and Biotechnology to a large class of over 50 students regularly. His research interests had been to find and discover new applications of pressure driven membrane processes for food, biotechnology and renewable energy. He was Principal Investigator of many sponsored projects worth Rs 3.5 crores from Government of India and private entrepreneurs. He published in Journals of Membrane, Bioseparation, Separation Science & Technology, Journal of Chromatography and Bioresource Technology