HYBRID EVENT: You can participate in person at Rome, Italy or Virtually from your home or work.

4th Edition of Euro-Global Conference on Biotechnology and Bioengineering

September 19-21 | Hybrid Event

September 19-21, 2024 | Rome, Italy
ECBB 2023

Pooja Kumari

Pooja Kumari, Speaker at Bioengineering Conferences
Amity University, India
Title: Structural insight into cellular regulation by human DND1 and associated conformational plasticity


RNA Recognition Motifs (RRMs) being the most abundant RNA binding domain in eukaryotes, is a major player in cellular regulation. Human DND1 (dead-end protein homolog1) also known as microRNA-mediated repression inhibitor 1 is an RNA-binding protein containing two RRMs in tandem. It is essential for the migration and viability of primordial germ cells during development, its complete loss causes lethality and mutations in the gene are associated with perturbed cellular regulation and eventually cancer. We have determined the 2.3 Å crystal structure of the human DND1 RRM2 domain. The structure revealed an interesting non-canonical RRM fold. This is maintained by the formation of a 3D domain-swapped dimer between β1 and β4 strands across protomers. We have delineated the structural basis of the stable domain-swapped dimer formation using the residue level dynamics of protein explored by NMR spectroscopy and MD simulations. Our results indicate that the monomer to dimer switch is governed by several determinants such as hinge loop residues, its length, hydrophobicity, the concentration of protein, disulfide bond, and so forth. Several variations in the canonical βαββαβ topology have been observed so far. However, this is the first report of domain swapping in the RRM domain which may increase the binding surface area and allow this protein to exhibit a multifaceted role during post-transcriptional regulation. Our structural and dynamics studies substantiate major determinants and molecular basis for domain-swapped dimerization observed in the RRM domain that could allow varied roles for its RRMs


Pooja Kumari studied B.Sc. and M.sc. at the Banaras Hindu University, Varanasi from 2011-2016. She then qualified national level fellowship CSIR-JRF and secured a Ph.D. position at the International Center for Genetic Engineering and Biotechnology, (ICGEB) New Delhi and joined the group of Dr. Neel Sarovar Bhavesh gaining critical knowledge on protein biochemistry, X-Ray crystallography and Nuclear Magnetic Resonance (NMR). She received her Ph.D. degree in 2021 from Jawaharlal Nehru University Delhi. Followed by which she did her postdoctoral studies at National Center for Biological Sciences, Bangalore. She obtained the position of Assistant Professor at the Amity Institute of Biotechnology, Amity University Jharkhand in 2022. She has published seven articles in SCI journals.