HYBRID EVENT: You can participate in person at Rome, Italy or Virtually from your home or work.

4th Edition of Euro-Global Conference on Biotechnology and Bioengineering

September 19-21 | Hybrid Event

September 19-21, 2024 | Rome, Italy
ECBB 2023

The lactic acid bacteria lactococcus lactis an surprisingly produce intracellular vesicles

Frelet Barrand Annie, Speaker at Bioengineering Conferences
Institute FEMTO, France
Title: The lactic acid bacteria lactococcus lactis an surprisingly produce intracellular vesicles

Abstract:

Membrane proteins (MPs) perform a wide variety of functions vital to the survival of organisms. Involved in numerous pathologies, they are important drug targets. In spite of their functional and biotechnological importance, their study remains difficult due to their hydrophobicity and low abundance in cells. Their overexpression in heterologous systems is mandatory for their detailed structural and functional characterization. However, this strategy leads to numerous obstacles such as their toxicity to hosts and the quality of the MP produced in these systems, especially for structural studies. An original approach to produce intracellular vesicles was tested using the ability of a small MP, caveolin 1β, to generate membrane vesicles within the cytoplasm when heterologously overexpressed. Such structures were observed in Escherichia coli and insect cells. The overexpression of caveolin 1β was tested in the lactic acid bacteria, Lactococcus lactis, since these bacteria appeared to emerge as a good alternative to E. coli and because they display a very different lipid composition. Surprisingly, L. lactis was able to produce intracellular vesicles with a size comparable to E. coli h-caveolae. Biochemical and biophysical studies have been carried out to realize a deeper characterization of such nanovesicles prior to other further applications of these nanovesicles

Audience Take Away Notes :

  • Eukaryotic and L. lactis use for production of nanovesicles will help people facing problems of expression of proteins of interest in classical expression systems, either prokaryotic or also give opportunities
  • To develop collaborations around nanovesicles production and characterization

Biography:

Dr. Annie FRELET-BARRAND studied biochemistry at the University of Franche-Comté (France) and was graduated as MS in 1998. In 2006, she received her PhD degree on MPs characterization at the Institute of Plant Biology, Zurich. During her postdoctoral fellowship (CEA Grenoble, France), she developed L. lactis system for functional characterization of MPs. In 2009, she became CNRS Researcher at CEA Saclay, studying MPs involved in liver detoxification. In 2015, she integrated the Institute FEMTO-ST and is now studying on biochemical and biophysical ways to study proteins and other biological elements. She published 19 research articles and 4 book chapters (h=14).

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