3rd Edition of Euro-Global Conference on Biotechnology and Bioengineering

June 14-15 | Online Event

June 14-15, 2023 | Online Event
ECBB 2023

Frelet Barrand Annie

Frelet Barrand Annie, Speaker at Bioengineering Conferences
Institute FEMTO, France
Title: The lactic acid bacteria lactococcus lactis an surprisingly produce intracellular vesicles


Membrane proteins (MPs) perform a wide variety of functions vital to the survival of organisms. Involved in numerous pathologies, they are important drug targets. In spite of their functional and biotechnological importance, their study remains difficult due to their hydrophobicity and low abundance in cells. Their overexpression in heterologous systems is mandatory for their detailed structural and functional characterization. However, this strategy leads to numerous obstacles such as their toxicity to hosts and the quality of the MP produced in these systems, especially for structural studies. An original approach to produce intracellular vesicles was tested using the ability of a small MP, caveolin 1β, to generate membrane vesicles within the cytoplasm when heterologously overexpressed. Such structures were observed in Escherichia coli and insect cells. The overexpression of caveolin 1β was tested in the lactic acid bacteria, Lactococcus lactis, since these bacteria appeared to emerge as a good alternative to E. coli and because they display a very different lipid composition. Surprisingly, L. lactis was able to produce intracellular vesicles with a size comparable to E. coli h-caveolae. Biochemical and biophysical studies have been carried out to realize a deeper characterization of such nanovesicles prior to other further applications of these nanovesicles

Audience Take Away Notes :

  • Eukaryotic and L. lactis use for production of nanovesicles will help people facing problems of expression of proteins of interest in classical expression systems, either prokaryotic or also give opportunities
  • To develop collaborations around nanovesicles production and characterization


Dr. Annie FRELET-BARRAND studied biochemistry at the University of Franche-Comté (France) and was graduated as MS in 1998. In 2006, she received her PhD degree on MPs characterization at the Institute of Plant Biology, Zurich. During her postdoctoral fellowship (CEA Grenoble, France), she developed L. lactis system for functional characterization of MPs. In 2009, she became CNRS Researcher at CEA Saclay, studying MPs involved in liver detoxification. In 2015, she integrated the Institute FEMTO-ST and is now studying on biochemical and biophysical ways to study proteins and other biological elements. She published 19 research articles and 4 book chapters (h=14).