Isolation and enzymatic characterization of microorganisms associated with municipal organic solid waste decomposition

Title: Isolation and enzymatic characterization of microorganisms associated with municipal organic solid waste decomposition

Abstract:

Municipal organic solid waste is a rich source of carbohydrates, proteins, fats and lignocellulosic waste which provides an environment for the growth of different waste decomposing microbes. In Indian scenario huge amount of waste is being generated per day. It becomes a huge environmental problem to dispose of this waste in big cities. Also it develops serious health issues among citizens in dumping areas. In the present study, the municipal organic solid waste decomposing microorganisms were isolated from three different waste dumping and processing sites from pune, Maharashtra. The waste degrading microbes were isolated by serial dilution method on nutrient agar and potato dextrose agar. 118 bacterial isolates and 20 fungal isolates were screened for extracellular enzyme production at different pH (5.5 and 9), temperature (37⁰C and 50⁰C), and desiccator conditions. Based on the primary screening 40 bacterial isolates and 6 fungal isolates were selected for in vitro relative enzyme activity of amylase, protease, cellulase and lipase using various growth mediums like starch agar, casein agar, tween-80 agar and carboxy methyl cellulose agar. The 16 bacterial and 6 fungal isolates showing relative enzyme activity of minimum 2 and producing multienzymes were studied for enzymatic characterization. Enzyme assay of selected microbes were performed at different pH, temperature and aeration conditions. The optimal growth conditions, morphological characters, antagonistic effect and biochemical characterization were performed for the selected isolates. The isolates showed significant enzyme production and absence of antagonistic effects among each other. Thus, these results have increased the scope of finding most suitable waste decomposing microbial consortium.  From the present investigation, it can be concluded that the extracellular multienzyme producing microbial consortium can be served as an ecofriendly source for bioconversion of municipal organic solid waste.

Keywords: Municipal organic solid waste, Waste decomposition, Microbial consortium, Enzymatic characterization.

Audience Take Away Notes:

• Composition of solid municipal waste
• It becomes a huge environmental problem to dispose of this waste in big cities
• Microbes can degrade the solid waste in to simple nutrients
• The extracellular multienzyme producing microbial consortium can be served as an ecofriendly source for bioconversion of municipal organic solid waste

Biography:

Prof Dr. Sunil Pawar is working as a professor in Microbiology at Department of Microbiology and P G research center T C college (Empowered Autonomous college) Baramati, affiliated to Savitribai Phule Pune University, Pune MS India presently He is working as a chairman in Microbiology Savitribai Phule Pune University, Pune MS India. He is a member of academic council as well as Faculty of science, Savitribai Phule Pune University, Pune MS India. He has completed four research students and seven research students are working with him. He has also completed one Post doctoral fellow.He has a teaching experience of 34 years at UG as well as PG level. He has completed three Major Research Projects and three Minor projects funded by various agencies of Govt. Of India. He has received Rs. 70, 00000 grants from different funding agencies in last fifteen years. He has published more than 20 papers in national and international reputed journals. He is actively participated in several national and international conferences.