Isolation and enzymatic characterization of microorganisms associated with municipal organic solid waste decomposition

Title: Isolation and enzymatic characterization of microorganisms associated with municipal organic solid waste decomposition

Abstract:

Municipal organic solid waste is a rich source of carbohydrates, proteins, fats and lignocellulosic waste which provides an environment for the growth of different waste decomposing microbes. In Indian scenario huge amount of waste is being generated per day. It becomes a huge environmental problem to dispose of this waste in big cities. Also it develops serious health issues among citizens in dumping areas. In the present study, the municipal organic solid waste decomposing microorganisms were isolated from three different waste dumping and processing sites from pune, Maharashtra. The waste degrading microbes were isolated by serial dilution method on nutrient agar and potato dextrose agar. 118 bacterial isolates and 20 fungal isolates were screened for extracellular enzyme production at different pH (5.5 and 9), temperature (37⁰C and 50⁰C), and desiccator conditions. Based on the primary screening 40 bacterial isolates and 6 fungal isolates were selected for in vitro relative enzyme activity of amylase, protease, cellulase and lipase using various growth mediums like starch agar, casein agar, tween-80 agar and carboxy methyl cellulose agar. The 16 bacterial and 6 fungal isolates showing relative enzyme activity of minimum 2 and producing multienzymes were studied for enzymatic characterization. Enzyme assay of selected microbes were performed at different pH, temperature and aeration conditions. The optimal growth conditions, morphological characters, antagonistic effect and biochemical characterization were performed for the selected isolates. The isolates showed significant enzyme production and absence of antagonistic effects among each other. Thus, these results have increased the scope of finding most suitable waste decomposing microbial consortium.  From the present investigation, it can be concluded that the extracellular multienzyme producing microbial consortium can be served as an ecofriendly source for bioconversion of municipal organic solid waste.

Keywords: Municipal organic solid waste, Waste decomposition, Microbial consortium, Enzymatic characterization.

Audience Take Away Notes:

• Composition of solid municipal waste
• It becomes a huge environmental problem to dispose of this waste in big cities
• Microbes can degrade the solid waste in to simple nutrients
• The extracellular multienzyme producing microbial consortium can be served as an ecofriendly source for bioconversion of municipal organic solid waste

Biography:

Dr. Sunil T Pawar studied Microbiology at the Shivaji University, Kolhapur, MS, India and graduated in 1989. he then joined the research group of Prof. Puranik Pravin at the School of Life Sciences North Maharashtra university Jalgaon MS India. He received his PhD degree in 2009 at the same institution. He has completed three major and two minor research projects funded by Govt. of India. He has more than 25 research publications on his own credit. He has guided three PhD students successfully and presently five students are working under his guidance. Presently He is a Chairman of Microbiology board of studies of Savitribai Phule Pune University, Pune (SPPU) and T C College, Baramati (Autonomous) He is a member of Academic council (SPPU). He is a member of College development committee at T C College Baramati Dist Pune India he obtained the position of Professor at the T C College (SPPU).