Production of nanoliposomal amphotercin B topical gel as effective treatment for human Cutaneous Leishmaniasis (CL) disease

Title: Production of nanoliposomal amphotercin B topical gel as effective treatment for human Cutaneous Leishmaniasis (CL) disease

Abstract:

Human cutaneous leishmaniasis (CL) is the disease caused by Leishmania sp. parasite and it endemic in Iraq and other world countries. This study comprised 78 cases of suspected CL disease. Before treatment, 66 (84.6%) cases were positive and 12 (15.4%) cases were negative among the three treatment groups. The reduction in the percentage of cases with positive microscopical examination was more pronounced in the case of Pentostam (Sodium Stibogluconate) during the first three weeks, but Nanoliposomal amphotericin B 0.4 % becomes more effective as the percent of cases with positive microscopical examination decreases.

Using the Nested-PCR reaction, the molecular technique revealed 60 (76.9%) positive and 18 (23.1%) negative examples from 78 skin lesion samples. The best results were found with Nanoliposomal amphotercin B 0.4 % topical gel, followed by pentostam 3 (20%), and finally flagyl 3 (12%) injections, when nested PCR exams were compared between the three therapy groups during weeks of follow up.

The study found a highly significant difference in mean recovery time between treatment groups (p < 0.001). The mean recovery time for the Nanoliposomal amphotericin B 0.4 % group was 2.87 ±0.67 (1-4) weeks, which was the shortest time ever documented. The mean recovery time in the flagyl group was 5.08 ±1.55 (1-8) weeks, which was the longest duration ever documented. The pentostam group had a mean recovery time of 4.00 ±1.13 (2-6) weeks, which was comparable to nanoliposomal amphotericin B0.4 % and flagyl.

In comparison to flagyl and pentostam groups, there was a significant change (reduction) in lesion size (cm) in the Nanoliposomal amphotericin B 0.4% group; however, changes in mean size were equivalent in the majority of weeks of follow up (p > 0.05). Lesion sizes shrank after three and four weeks.
Keywords:  Leishmaniasis, Human, Diagnostic methods, Kala-azar, PCR methods, Nanobiotechnology

Biography:

Prof. Dr. Abdulsada Abdulabbas Rahi is currently working as Dean of Science College of Wasit University, Iraq. Dr Rahi received his Postdoctoral degree on Medical Parasitology from the University of Tehran of Medical Sciences (TUMS), Iran. Dr Rahi completed his PhD on Biotechnology of Leishmaniasis from Al-Nahrain University, Iraq. Dr Rahi completed his Masters on Medical Microbiology from the University of Al-Anbar , Iraq. He then worked at the Institute Wasit University/ Iraq, served as Professor at the University in Medical Biotechnology. Dr. Rahi has authored several publications (67 Published papers) in various reputed journals. His publications reflect his research interests in Biotechnology and Medical Parasitology. Dr. Rahi is also an Associate Editor of the Journals:  Journal of Applied Sciences and Research, Journal of Agri-Food and Applied Sciences (JAAS), Journal of Scientific Research and Studies and Scholars Academic and Scientific Journals (SAS).
Dr. Scientist is serving as a member or fellow in Association of American Society for Microbiology (ASM). He is currently in charge of ongoing scholarly project Nanobiotechnology. Dr. Rahi is awarded Golden Prize by 10th International Exihibition of Inventions and Innovation Forum and 3rd World Inventions and Innovation Forum in China / Foshan and in SiriLanka, 2020. Dr. Rahi is awarded Golden Prizes from Germany 2021,2023 and INTARG Poland 2023, 2024. Also, Dr. Rahi is awarded Scientific Day's Prize for Medical Researchs, 2014 by Iraqi Ministry of Higher Education and Scientific Research. Dr. Rahi is awarded Silver Prize by International Exihibition of Inventions and Innovation Forum in Germany / Nuremberg, 2021. Dr. Rahi is supervised PhD and MSc students and Scientific referee.
Research Interest: I am interested in Molecular parasitology, Nanobiotechnology, Biotechnology of Leishmaniasis, Medical Parasitology, Medical Microbiology, Diagnostic methods, Kala-azar etc.