3rd Edition of Euro-Global Conference on Biotechnology and Bioengineering

June 14-15 | Online Event

June 14-15, 2023 | Online Event
ECBB 2023

Rakesh Kumar Verma

Rakesh Kumar Verma, Speaker at Biotechnology conference
Mody University of Science and Technology, India
Title: Molecular characterization and plant growth promotion potential of endophytes from Thar desert, Rajasthan India


The Great Indian Desert, also known as the Thar Desert, covers approximately 0.32 million km2 of the planet's surface, or 10% of India's whole geographic area. Despite being one of the smallest deserts in the world, it is home to a variety of habitats and species. One of the most challenging environments for plant growth is the desert ecosystem, where environmental challenges such water scarcity, extreme heat, salt, and irradiance limit plant diversity and dispersion. Due to its limited rainfall and high temperatures, Rajasthan's Thar Desert is one of the driest places on earth. Desert plants have developed a variety of stress tolerance techniques to get through their entire life cycle. Endophytes are defined as symbiotic bacterial and fungal communities that are found in intercellular and intracellular spaces of plants such as stem, root, leaves etc. and do not cause any negative impact on plant. The present research based on the sample collection from Thar desert of Rajasthan including Bikaner, Jaisalmer, Churu and Barmer districts of Rajasthan. The plants Leptadenia pyrotechnica, and Balanites aegyptiaca, were used for isolation of bacterial and fungal endophytes. Various bacterial and fungal species named Bacillus sp., Enterobacter sp., Pseudomonas sp., Sphingomonas sp., Aspergillus sp., Penicillium sp., Chaetomium sp., Trichoderma sp., Alternaria sp., Fusarium sp., Nigrospora sp., were isolated from these plants. Further these isolates were screened for their Plant Growth Promotion Activities such as nitrogen fixation, phosphate solubilization, HCN production, synthesis of siderophore, 1-aminocyclopropane-1-carboxylate (ACC) deaminase and extracellular enzyme production (amylase, chitinase, cellulase, protease, and catalase). These features of endophytic bacteria and fungi that encourage plant growth increase root length and density, which increases plant resistance to drought. Additionally, plant-endophytic bacteria help plants resist drought stress by creating a variety of volatile chemicals, indole-3-acetic acid, abscisic acid, and other drought-tolerant molecules. Indirectly, endophytic bacteria help inoculated plants' osmotic adjustment, relative water content, and antioxidant activity. The combined effects of this bacterial-mediated drought resistance and plant growth promotion mechanisms result in improved plant growth promotion and yield even under extreme drought circumstances.

Audience take away notes:

  • The endophytes (bacteria and fungi) isolated in this project have the potential to be employed as biocontrol agents or biofertilizers on other agricultural crops, including wheat, cowpea, guar, etc., to increase crop yields and improve the plants' resistance to biotic and abiotic stress.
  • Endophytes, isolated from this study, are a possible source of metabolites or bioactive chemicals for sustainable agriculture, medical industry having the ability to fight against crop diseases.
  • The use of natural control agents, such as endophytes and their metabolites, makes them more ecologically preferable than pesticides.
  • Further  characterization of defense genes like SOD, PAL, and catalase etc. will provide a great understanding of the involvement of  endophytes in stress management in plants.


Rakesh Kumar Verma has been studying the rinteraction between plants and microbes since 2012. His doctoral dissertation focused on the evolution of virus genomes in plants. Dr. Verma earned his Master of Science in Biotechnology from Jaipur National University in Jaipur, India, in 2011. Afterward, he became a DBT Research fellow in the lab of Prof. R.K. Gaur at Mody University, where he ultimately earned his Ph.D. Subsequently, he began working in 2018 as an assistant professor at Mody University. In 2015, he was inducted into ISCA and AMI as a life member after being honored as a young scientist at a national conference. He has more than 30 research papers published in high-impact, SCOPUS-indexed publications.