Title: Screening the antifungal and antibacterial activity of biobased coating made from laccase enzyme produced by marine actinomycetes
Due to the emergence of the antibiotic resistance of pathogenic bacteria and the side effects often associated with synthetic chemicals, antimicrobial compounds extracted from herbs, plants and microbes have been widely investigated and used as a sustainable and ecofriendly solution. Laccases are multicopper-containing oxidases (EC22.214.171.124.) widely found in higher Plants and Microbes. Because of their high pH stability and thermostability, laccases have potential applications in textile, plastics and paper industries. This study involved examining the possibility of using the biobased coating made from laccase enzyme produced by a Marine Actinomycetes strain as a potential Antimicrobial for the plastics and paper industries. Laccase activity was confirmed by plate assay technique using 5mM Guaiacol. The Antibacterial Activity of coated plastics chip and paper with Laccase enzyme ATCC 6205, Aurobasidium pullulans ATCC 15233 as per ASTM G21 which showed the treated showed 99.99% reduction of S. aureus ATCC 6538 and E. coli ATCC 8739 when tested in accordance with ISO 22196:2011. The Antifungal activity was studied against Aspergillus niger ATCC 9642, Penicillium funicolosum ATCC 11797, Gliocladiu m virens ATCC 9645, Chaetobium globosum samples were resistant to fungal attack at the end of 28 days of incubation.
Keywords: Laccase, Biobased coating, Actinomycetes, Antibacterial, Antifungal, Plastic and paper industry, Sustainable, Ecofriendly, ISO 22196, ASTM G21
Audiece Take Away Notes :
- Plastics, paper and textile industries can evaluate the research and plan for trails on their products.
- Help move to innovative solutions for a sustainable product for betterment of the future.
- Yes, this provide a practical solution to a problem that could simplify or make a designer’s job more efficient.
- Yes, it can improve the accuracy of a design, or provide new information to assist in a design problem