HYBRID EVENT: You can participate in person at Rome, Italy or Virtually from your home or work.

4th Edition of Euro-Global Conference on Biotechnology and Bioengineering

September 19-21 | Hybrid Event

September 19-21, 2024 | Rome, Italy
ECBB 2024

Ishika Jain

Ishika Jain, Speaker at Biotechnology Congress 2024
Amity University Lucknow, India
Title: RNA extraction and quantification from blood


Extraction and quantification of RNA from blood is important for various RNA based studying RNA structure and function, invitro translation, Northern blotting. There are various methods to isolate or extract RNA from blood using different RNA extraction kit i.e. RNeasy(minikit) and Ribopure (RNA purification kit -blood). Other conditions are also very crucial for isolation of high-quality RNA such as storage temperature. After isolation and treatment of RNA with DNase, the quality of RNA is assessed by nano drop and gel electrophoresis. RNA is structurally different from DNA, so methods of DNA extraction are not applied directly from RNA extraction. RNA is generally single stranded while in most cases DNA is double stranded. Extraction or isolating intact RNA is often a difficult procedure. For RNA extraction, maintenance of aseptic environment is necessary as the major cause which affects integrity of RNA is presence of ribonucleases. For downstream application of extracted RNA, it is crucial to get high quality and quantity of extracted RNA


Ishika Jain is from Amity University Lucknow, India.