Title: RNA extraction and quantification from blood
Abstract:
Extraction and quantification of RNA from blood is important for various RNA based studying RNA structure and function, invitro translation, Northern blotting. There are various methods to isolate or extract RNA from blood using different RNA extraction kit i.e. RNeasy(minikit) and Ribopure (RNA purification kit -blood). Other conditions are also very crucial for isolation of high-quality RNA such as storage temperature. After isolation and treatment of RNA with DNase, the quality of RNA is assessed by nano drop and gel electrophoresis. RNA is structurally different from DNA, so methods of DNA extraction are not applied directly from RNA extraction. RNA is generally single stranded while in most cases DNA is double stranded. Extraction or isolating intact RNA is often a difficult procedure. For RNA extraction, maintenance of aseptic environment is necessary as the major cause which affects integrity of RNA is presence of ribonucleases. For downstream application of extracted RNA, it is crucial to get high quality and quantity of extracted RNA
