Title: CRISPR/Cas9 mediated PROZ gene knockout for creating animal model of Preeclampsia
Abstract:
The complicated pregnancy disorders threaten the life of mothers and their children each year. Preeclampsia is one of the pregnancy disorders associated with hypertension. It affects 8% of pregnancies worldwide. According to a number of factors, such as hypertension, urine protein concentration, symptomatology, and age of onset of the disease it is classified at mild and severe levels. In severe disease, phenotypes such as red blood cell breakdown, a low blood platelet count, impaired liver function, kidney dysfunction, swelling, shortness of breath due to fluid in the lungs, and / or visual disturbances are visible. The studies conducted recently have uniquely focused on pathological mechanisms of placenta on disease incidence. The development of cellular and animal models has been considered by researchers for basic studies, enhancing cellular and molecular information and evaluating the underlying mechanisms of this disease. In the present study, our purpose is to develop gene editing in animal models and optimize Gene Editing methods for hypertensive disorders of pregnancy. Because of the role of PROZ gene in pregnancy complications and its relationships with the inflammation system, it may be a good candidate for studying the interactions of coagulation and inflammation systems. We attempted to examine the ability of gene editing through CRISPR / Cas9 first in the cell line trophoblast and its efficacy for mice with knockout PROZ gene and evaluate new therapeutic strategies by developing new mice models
