Title: Neuroprotective effect of RMF in sporadic parkinson’s disease model mice
Abstract:
Progressive loss of dopaminergic neurons in Substantia Nigra Par Compacta (SNpc) due to aggregation of Lewy bodies is the hallmark of Parkinson’s disease (PD). ROS plays a key role in formation of Lewy bodies, thus leading to mitochondrial dysfunction and apoptosis of neurons. Rotating magnetic field (RMF) is an emerging non-invasive technique for prevention of neurodegenerative disorders. To investigate the potential therapeutic effects of RMS in PD, we subjected an experimental mouse model to RMF. CblC mice were injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) (30 mg/kg, i.p., once daily for 5 days) followed by RMF treatment at a frequency and intensity of 4 Hz and 0.4 T respectively. The daily 2 hours treatment with RMF was continued for a period of 6 months. We examined the effect of RMF on motor functions, aggregation of Lewy bodies and the integrity and viability of total and dopaminergic neurons in SNpc and striatal regions. We further performed the transcriptomic analysis of SNpc tissue of PD versus the SHAM mice. Our results showed that exposure to RMF improved the motor functions, enhanced neuronal cell viability and protected their integrity in PD mouse model. We further showed that RMF diminishes the aggregated Lewy bodies in the neurons and reduces ROS production. The whole transcriptomic analysis uncovered that RMF promotes the expression of anti-apoptotic versus the pro-apoptotic genes specifically involved in mitochondrial apoptosis.