Title: Protective effect of ginkgo biloba extract against diclofenac induced nephrotoxicity
The objective of the present study was to evaluate the ability to orally administration of Ginkgo biloba (G.b) extract against kidney damage induced by diclofenac (DF) in male rats with different doses and times The experimental rats were divided into six groups that further divided to 6 subgroups. The first group was served as control, the second group was given G.b extract orally at a dose of 400 mg/kg b.wt/day using the gastric tube for 7 or 14 days. The third group was injected i.m. once/day DF at a dose of 10 mg/kg b.wt. Then, animals injected i.m DF at a dose of 10 mg/kg b.wt/daily and received orally freshly prepared G.b extract at a dose of, 100 mg/kg, 200 mg/kg, and 400 mg/kg b.wt for 7 or 14 days. Administration of DF induced adverse effects on the kidney functions estimated parameters such as creatinine, urea, and uric acid concentration, disturb oxidative stress and antioxidant markers (MDA, GSH, SOD, CAT, and GPx), as well as abnormal in anti-inflammatory markers, prostaglandin, TNF-a, IL-1 β and IL-6. The administration of G.b extract withdifferent doses (100, 200, 400 mg/kg) for 7 or 14 days suppresses the renal injury induced by DF and keeps 2 the kidney functions nearly within the normal limit. The most effective protective role was recorded at the highest dose and longer time.