3rd Edition of Euro-Global Conference on Biotechnology and Bioengineering

June 14-15 | Online Event

June 14-15, 2023 | Online Event
ECBB 2023

Bushra Muneer

Bushra Muneer, Speaker at Bioengineering Conference
Institute of Industrial Biotechnology, Pakistan
Title: Role of anoxybacillus Sp. copper processing gene in the removal of copper from wastewater


Resistant thermophilic strain was isolated from wastewater and recognized as Anoxybacillus rupiensis on the basis of 16S rRNA gene sequence analysis. It was grown in LB media at 60oC with pH 7.0. It showed typical growth pattern except for the lag phase extended in the presence of copper because of resistance against metal. An efflux system plays an important role in pumping out of metal. Therefore, the effects of cu resistance have been thoroughly investigated. SEM and FT-IR were used to analyze changes in the surface macrostructure and functionality of this strain showing interaction with Cu. The ability to adsorb and absorb Cu2+ inside their cells was evaluated by AAS, which showed that bacterial cell had the ability to adsorb 72% of the Cu2+ and absorb 28% Cu2+ inside them. Expression of copper resistant gene was quantified through PCR by adding primers. The result of purified PCR product demonstrated that copper resistant gene was functional. This is the first research to identify copA gene in thermophilic A. rupiensis. It is concluded that copper-resistant gene copA proving that bacteria could make much more efficient inoculum for remediation of copper in contaminated industrial wastewater