Yash Saini

Title: The utilization of click reactions in understanding the structure and function of IgE in allergic reactions

Abstract:

This study examines cutting-edge techniques, namely click chemistry and antigen-antibody complex formations, in order to develop affordable and effective home allergy testing kits. The ultimate objective is to provide effective methods for diagnosing and treating allergic conditions. Through an azide click reaction, the surface of the hydrophilic polymer-coated paper is functionalized with captured antibodies and used as a diagnostic substrate for the detection of food allergies. After introducing a blood sample and a second antibody conjugated with a detection moiety, the presence of specific IgE antibodies to the food allergen is determined by measuring the fluorescence or color intensity of bound IgE. By comparing the test's results to internationally recognized benchmarks, the test's accuracy is confirmed. When examining how IgE interacts with antigens and its role in effector cell activation, the application of click chemistry in this context has substantial implications for developing novel therapies for allergic diseases. Additional research in this field will increase our understanding of the underlying mechanisms, paving the way for future advances in allergy diagnosis and treatment

Audience Take Away Notes :

• Understand new methods of click chemistry for the rapid detection of food-based allergies on a paper assay
• Plethora of researchers will be encouraged to develop sophisticated yet rapid methods for allergy testing that can be provided at a low cost and with a user-friendly model
• This gives a more practical approach to the initiative of distributing/reaching out to the people for a better solution for rapid allergy testing. The allergy detection methods currently present require professional medical assistance and a cost-effective model

Biography:

Yash Saini is a student at BITS Pilani K K Birla Goa Campus studying B. Tech Chemical and M.Sc. Chemistry. He has worked in various fields of Biotechnology, such as BCI, drug delivery systems, and Immunological responses.  He is willing to pursue his thesis in BCI in AI/ML in the coming future. He has also worked on numerous projects, including making EEG component circuits using OTAs instead of op-amps, as OTA gives the ability to achieve voltage control of the center frequency and Q of the active filter from the tunable transconductance of the OTA. Designing a rover with allowed motion in all 4 directions, with an attached robotic claw that can be controlled by SSVEP signals from the brain to lift and move light objects through a brain-computer interface. Along with developing a rapid allergy testing kit using click chemistry on a paper assay.