Proteins are crucial biomolecules involved in numerous biological processes, making their purification and determination essential for scientific research and biotechnological applications. This abstract provides an overview of protein purification methods and protein determination techniques, emphasising their significance in modern scientific investigations. The process of protein purification involves isolating a target protein from complex biological mixtures, such as cell lysates or tissue extracts, while eliminating unwanted contaminants. Purified proteins serve as invaluable tools for functional characterisation, structural studies, and proteomic analysis. The purification process employs diverse techniques, including chromatography (size exclusion, ion exchange, and affinity), precipitation, electrophoresis, ultrafiltration, and dialysis. Each technique exploits specific physical and chemical properties of proteins, allowing their efficient separation and concentration. Once purified, accurately determining the concentration of the target protein is essential for subsequent experiments and applications. Various methods for protein determination are available, such as spectrophotometric assays, the Bradford assay, Lowry assay, and BCA assay. These techniques rely on measuring colorimetric changes, absorbance, or fluorescence properties of proteins, providing quantitative data for accurate protein concentration determination. In this abstract, we highlight the importance of protein purification and determination in scientific research, elucidating protein functions, studying protein structures, and advancing biotechnological innovations. Researchers and biotechnologist rely on purified proteins as tools for drug development, diagnostics, and the production of recombinant proteins. Moreover, purified proteins are critical in proteomic studies, where their identification and quantification drive advancements in understanding cellular processes. This abstract emphasises the significance of protein purification and determination as essential steps in modern biochemistry and molecular biology. Understanding these processes provides a solid foundation for researchers to explore the diverse functions and properties of proteins, leading to significant breakthroughs in the fields of medicine, biotechnology, and beyond.